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Research in Vestibular Science > Volume 8(0); 2009 > Article
Research in Vestibular Science 2009;8(0): 51-57.
Colocalization of 5-HT1F Receptor and Calcitonin Gene-Related Peptide in Rat Vestibular Nuclei
Seong-Ki Ahn, Roza Khalmuratova, Sea-Yuong Jeon, Jin-Pyeong Kim, Jung Je Park, Dong Gu Hur
Department of Otolaryngology, School of Medicine, Gyeongsang National University, Jinju, Korea
Colocalization of 5-HT1F Receptor and Calcitonin Gene-Related Peptide in Rat Vestibular Nuclei
Seong-Ki Ahn, Roza Khalmuratova, Sea-Yuong Jeon, Jin-Pyeong Kim, Jung Je Park, Dong Gu Hur
Department of Otolaryngology, School of Medicine, Gyeongsang National University, Jinju, Korea
ABSTRACT
Background and Objective:   The frequent co-occurrence of migraine and balance disorders suggests a pathophysiologic link between the two. 5-HT1F receptor agonists and CGRP receptor antagonists have recently attracted attention as potential treatments for migraine, and CGRP release from trigeminal neurons has been associated with migraine. The aim of this study was to determine whether CGRP colocalizes with 5-HT1F receptor in rat vestibular nuclei using a double immunohistochemical staining procedure.
Materials and Methods:  
Male S-D rats were used in this study. Double immunofluorescence was employed to identify the colocalization of 5-HT1F receptor and CGRP in vestibular nuclei using alternating brainstem sections. In addition, other sections of rat brain were processed to visualize the cellular localizations of 5-HT1F receptor and CGRP in vestibular nuclei, so as to allow comparisons of the regional distributions of 5-HT1F receptor and CGRP. In order to perform semi-quantitative analysis on doubly labeled and singly labeled neurons, 4 randomly selected areas were chosen. Stained cells were counted in the superior vestibular nucleus, the medial vestibular nucleus, the lateral vestibular nucleus, and the spinal vestibular nucleus.
Results:  
Immunopositive neurons for 5-HT1F receptor and CGRP were immunopositive neurons were widely distributed throughout the 4 major vestibular nuclei, respectively. 5-HT1F receptor and CGRP co-expression was observed in the 4 major vestibular nuclei.
Conclusions:  
This study suggests that 5-HT1F receptor regulates the release of CGRP from vestibular nuclei. This finding indicates that 5-HT1F receptor agonists may ameliorate migrainous vertigo by attenuating elevated levels of CGRP release from vestibular nuclei.
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