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J Korean Bal Soc > Volume 5(1); 2006 > Article
Journal of the Korean Balance Society 2006;5(1): 35-43.
신생 흰쥐 난형낭의 조직배양 모델 군에서 저출력 레이저에 의한 전정유모세포 재생 증진 효과에 대한 연구
, , , , , 정용원1, 안진철2, 임은석1, 김영생1, 이민영1, 정재윤1, 이정구1,2
단국대학교 의과대학 이비인후-두경부외과학교실1, 의학레이저연구센터2
A Promotive Effect of Low Level Laser on Hair Cell Viability in Postnatal Organotypic Culture of Rat Utricles
Sun Young Oh, Kwang Dong Choi, Jae Moon Kim, Jei Kim, Seong Ho Park, Ji Soo Kim
1Department of Neurology, Chungnam National University College of Medicine, Daejeon, Korea.
2Department of Neurology, Seoul National University College of Medicine, Seoul,Korea.jisookim@snu.ac.kr
3Department of Neurology, Pusan National University College of Medicine, Busan, Korea.
ABSTRACT
Background and Objectives:   To culture and maintain mammalian hair cells is still a big challenge. In this study, long-term organotypic culture of rat utricular maculae was established to study vestibular hair cell. The effects of low level laser on hair cell viability in postnatal organotypic culture of rat utricles were investigated.
Materials and Method:   Uticular explants were prepared from postnatal 2 to 7 rats and cultured. To improve hair cell survival, the utricles were irradiated daily with low level laser. Whole-mount utricles were stained with FM1-43 which is known to be an efficient marker to identify live hair cells in cultured tissues. Such cells visualized directly through tissue culture dish with cover glass bottom by Confocal laser scanning microscope at specific time points.
Results:   The explanted utricular hair cells were cultured for up to 31 days in in vitro culture system. In low level laser irradiation group, utricular hair cells were more survived at 24 DIV and 31 DIV.
Conclusion:   These results suggest that low level laser promotes hair cell viability in utricular explants. Key Words : Organotypic culture, Low level laser, FM 1-43, Utricle
Keywords: Head-shaking nystagmus; Cerebellum; Vestibule-ocular reflex
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