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J Korean Bal Soc > Volume 2(1); 2003 > Article
Journal of the Korean Balance Society 2003;2(1): 86-94.
흰쥐에서 Arsanilate에 의한 일측 전정기관 손상 후 뇌간신경핵 c-Fos 단백발현의 시공간적 변화
, , , , , , 김재효1, 최창익1, 이문영1, 김민선1, 소병수1, 박은호2, 박병림1
1원광대학교 의과대학 생리학교실 및 전정와우기관연구센터 2베스트이비인후과
Spatio-temporal Changes on c-Fos Protein Expression in the Brain Stem Nuclei following Arsanilate-induced Unilateral Labyrinthectomy in Rats
Jae Hyo Kim, Chang Ig Choi, Moon Young Lee, Min Sun Kim, Byung Soo Soh, Eun Ho Park, Byung Rim Park
1Department Physiology, Wonkwang University School of Medicine and Vestibulocochlear Research Center at Wonkwang University, Iksan 570-749, Korea. byungp@wonkwang.ac.kr
2Best ENT's Clinic, Kwangju 503-310, Korea.
ABSTRACT
Spatio-temporal changes on c-Fos protein expression were investigated in vestibular compensation following unilateral labyrinthectomy (UL) induced by injection of arsanilate into the middle ear cavity, chemical labyrinthectomy, or surgical labyrinthectomy in medial vestibular nuclei (MVN), prepositus hypoglossal nuclei (PrH), and inferior olivary nuclei (ION) of Sprague-Dawley rats. Number of spontaneous nystagmus in surgical labyrinthectomy group was 28.2±1.2 beats/10 sec at post-op 2 hs and the nystagmus disappeared 76 hs after UL. In chemical labyrinthectomy group, spontaneous nystagmus occurred 6 hs after UL and increased up to maximum at 12 hs and disappeared 96 hs. Head deviation in surgical labyrinthectomy group reached a peak at post-op 2 hs and recovered to control level at 144 hs, but chemical labyrinthectomy produced head deviation 24 hs after UL and increased degree of the deviation over time till 144 hs. Expression of c-Fos protein in surgical labyrinthectomy group at post-op 2 hs was 81±19.4 cells in ipsilateral MVN to the lesion side and 212±60 cells in contralateral MVN, which showed severe asymmetry between bilateral MVN, and decrease of c-Fos protein expression was more in contralateral MVN than in ipsilateral MVN at 6 hs. Chemical labyrinthectomy expressed more c-Fos protein in contralateral MVN 6 hs after UL and in ipsilateral MVN 12 hs after UL, which showed asymmetry of c-Fos protein expression between bilateral MVN. And the expression in ipsilateral MVN of chemical labyrinthectomy group was increased gradually 48 hs after UL and reached a peak at 72 hs. In chemical labyrinthectomy group, expression of c-Fos protein in PrH was increased more in ipsilateral than in contralateral 6 hs after UL and more in contralateral 12 hs after UL, and ION showed more expression of c-Fos protein in contralateral than in ipsilateral 6 hs after UL through 72 hs. These results suggest that the course of vestibular compensation and the temporal expression of c-Fos protein in the brain stem nuclei following UL differed between surgical and chemical labyrinthectomy.
Keywords: Vestibular Compensation; c-Fos; Sodium arsanilate; Medial vestibular nucleus; Prepositus hypoglossal nucleus; Inferior olivary nucleus
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