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Research in Vestibular Science > Accepted Articles
Application of tetrode technology for analysis of changes in neural excitability of medial vestibular nucleus by acute arterial hypotension.
Young Kim2, Ho Koo3, Byung Rim Park1, Se Jin Moon3, Seung-Bum Yang4, Min Sun Kim1
1Wonkwang University School of Medicine, Iksan , Korea
2Department of Emergency Medicine, Wonkwang University Hospital, Iksan, Korea
3Department of Physiology, Wonkwang University School of Medicine, Iksan, Korea
4Department of Medical Non-commissioned Officer, Wonkwang Health Science University, Iksan, Korea
Correspondence  Min Sun Kim ,Tel: 82-63-850-6779, Fax: 82-63-852-6108, Email: mskim@wku.ac.kr
Received: September 16, 2018;  Accepted: October 24, 2018.  Published online: October 24, 2018.
ABSTRACT
Objectives &
Method:   Excitability o medial vestibular nucleus (MVN) in the brainstem can be affected by changes in the arterial blood pressure. Several animal studies have demonstrated that acute hypotension results in the alteration of multi-unit activities and expression of cFos protein in the MVN. In the field of extracellular electrophysiological recording, tetrode technology and spike sorting algorithms can easily identify single unit activity from multi-unit activities in the brain. However, detailed properties of electrophysiological changes in single unit of the MVN during acute hypotension have been unknown. Therefore, we applied tetrode techniques and electrophysiological characterization methods to know the effect of acute hypotension on single unit activities of the MVN of rats.
Results:   Two or three types of unit could be classified according to the morphology of spikes and firing properties of neurons. Acute hypotension elicited 4 types of changes in spontaneous firing of single unit in the MVN. Most of these neurons showed excitatory responses for about within 1 minutes after the induction of acute hypotension and then returned to the baseline activity 10min after the injection of sodium nitroprusside. There was also gradual increase in spontaneous firing in some units. In contrast small proportion of units showed rapid reduction of firing rate just after acute hypotension.
Conclusion:   Therefore, application of tetrode technology and spike sorting algorithms is another method for the monitoring of electrical activity of vestibular nuclear during acute hypotension.
Keywords: Medial vestibular nucleus; Acute arterial hypotension; Tetrode electrode; Spike sorting; Neuronal firing
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