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J Korean Bal Soc > Volume 4(2); 2005 > Article
Journal of the Korean Balance Society 2005;4(2): 243-249.
겐타마이신 독성이 유발된 전정 신경 세포주(UB/UE-1)에서 Caveolin-1의 발현
, , , , 조병한1, 김규성1, 김민욱1, 김민선2, 박병림2
인하대학교 의과대학 이비인후-두경부외과학교실1, 원광대학교 의과대학 생리학교실2
Expression of Caveolin-1 in the Differentiated Vestibular Cell Line (UB/UE-1) after Gentamicin Toxicity
Byung Han Cho, Kyu Sung Kim, Min Wook Kim, Min Sun Kim, Byung Rim Park
1Department of Otolaryngology-Head & Neck Surgery, Inha University College of Medicine, Incheon, Korea. stedman@inha.ac.kr
2Department of Physiology, Wonkwang University school of Medicine, Iksan, Korea.
Background and Objectives:   The caveolin is known as a mediator of cell death or survival of injured cell and inhibitor of various signaling pathways. We examined expression of caveolin-1 involved by protein kinase A(PKA) signaling pathway in the differentiated mouse vestibular cell line(UB/UE-1) after gentamicin toxicity.
Materials and Method:   We observed caveolae in the vestibular hair cell of healthy guinea pig through electron microscope. UB/UE-1 cells were cultured at 95% CO2, 5% O2, 33℃ for 2days and at 95% CO2, 5% O2, 39℃ for 24 hours for differentiation. Cells were treated with 1 mM of gentamicin, 0.02 mM H89 (PKA inhibitor), and then incubated for 24 hours. Caveolin-1 expression was examined by western blot and PKA activity by PepTagⓇ assay.
Results:   Caveolae were observed in the vestibular hair cell of healthy guinea pig by electron microscope. Caveolin-1 was expressed spontaneously in differentiated UB/UE-1 cells and increased after gentamicin treatment. PKA is overactivated by gentamicin treatment. The gentamicin induced caveolin-1 expression and PKA overactivation was inhibited by H89.
Conclusion:   Our results indicate that gentamicin induced caveolin-1 expression is mediated by PKA signaling pathway. We conclude that the caveolae/caveolin through a PKA signaling pathway is the important mechanism of gentamicin induced ototoxicity.
Keywords: Caveolin; Gentamicin; Ototoxicity
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